CBX5 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9876M-50, BS9876M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P45973

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB: 1:1000-1:5000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100

Background: Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and contain a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated at Lys9, a histone "mark" closely associated with centromeric heterochromatin.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of CBX5 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CBX5 Rabbit mAb at 1:1000 dilution
Lane 1: MCF7 whole cell lysate
Lane 2: Hela whole cell lysate, Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CBX5 antibody. Counter stained with hematoxylin., ICC staining CBX5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde,permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Chromobox protein homolog 5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha; HP1α; CBX5; HP1A

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified