DHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-EL-0016-24T, E-EL-0016-48T, E-EL-0016-96T

Citations, Manuals and MSDS Available upon request.

Abbreviation: DHVD3

Research Areas: Metabolism, Developmental biology

Target Species: Universal

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of DHVD3 concentrations in serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 4.69 pg/mL

Detection Range: 7.81-500 pg/mL

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal DHVD3. During the reaction, Universal DHVD3 in the sample or standard competes with a fixed amount of Universal DHVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal DHVD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal DHVD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Universal DHVD3 in samples. No significant cross-reactivity or interference between Universal DHVD3 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 12 months

Shipping: ice bag