MNT Polyclonal Antibody

Catalogue Numbers: BS5798-50, BS5798-100

Sizes: 50µl, 100µl

Alternative Name: Max-binding protein MNT; Class D basic helix-loop-helix protein 3; bHLHd3; Myc antagonist MNT; Protein ROX; BHLHD3; ROX

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99583

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Mnt (Max binding protein), also known as MAD6, ROX, bHLHd3 (class D basic helix-loop-helix protein 3) or MXD6, is a 582 amino acid nuclear protein that forms a complex with Max (Myc-associated factor X) to repress transcription. Mnt contains one basic helix-loop-helix (bHLH) domain and is encoded by a gene that maps to human chromosome 17p13.3. Chromosome 17 comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MNT polyclonal antibody detects endogenous levels of Max-binding protein MNT.

Bioworld Molecular Weight: ~ 62 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MNT polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 357-402 of Human MNT.

Conjugate: Unconjugated

Modification: Unmodified