NIP7 Polyclonal Antibody

Catalogue Numbers: BS60088-50, BS60088-100

Sizes: 50µl, 100µl

Alternative Name: 60S ribosome subunit biogenesis protein NIP7 homolog; KD93; NIP-7; CGI-37; HSPC031; HSPC180; OK/SW-cl.76; OK/SW-cl.78

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y221

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Nip7 is a nucleolar protein involved in ribosome biogenesis, specifically 27S pre-rRNA processing and 60S ribosome subunit assembly in Saccharomyces cerevisiae. NIP7 is a conserved protein among eukaryotes, including human, mouse, rat and pig that is essential for cell growth. In humans, NIP7 interacts with the Shwachman-Bodian-Diamond syndrome (SBDS) protein, which mediates accurate gene expression essential for proper brain, skeletal, and blood cell development. Mutations in the SBDS gene results in an autosomal disorder (SDS) characterized by pleiotropic phenotypes including pancreatic, skeletal and bone marrow deficiencies and predisposition to hematological dysfunctions.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NIP7 polyclonal antibody detects endogenous levels of NIP7 protein.

Bioworld Molecular Weight: ~ 20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of NIP7 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: H9C2 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human NIP7.

Conjugate: Unconjugated

Modification: Unmodified