MED9 Polyclonal Antibody

Catalogue Numbers: BS60176-50, BS60176-100

Sizes: 50µl, 100µl

Alternative Name: Mediator of RNA polymerase II transcription subunit 9; Mediator complex subunit 9; MED9; MED25

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NWA0

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: In mammalian cells, transcription is regulated in part by high molecular weight co-activating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II). The mediator complex is one such multi-protein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med9 (mediator complex subunit 9), also known as MED25, is a 146 amino acid nuclear protein and component of the mediator complex. Med9 directly interacts with Med4 and is encoded by a gene that maps to human chromosome 17p11.2. Chromosome 17 comprises over 2.5% of the human genome, encodes over 1,200 genes and is associated with two key tumor suppressor genes, namely, p53 and BRCA1.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MED9 polyclonal antibody detects endogenous levels of MED9 protein.

Bioworld Molecular Weight: ~ 20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MED9 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human MED9.

Conjugate: Unconjugated

Modification: Unmodified